Opportunity Overview
The Defense Health Agency Contracting Activity (DHACA) in Fort Detrick, MD intends to negotiate on a sole source basis (IAW RFO 12.201-1) with Illumina, Inc 5200 Illumina Way San Diego, CA 92122, as the only responsible source that can provide an Illumina NextSeq 2000 Sequencing System to replace the Illumina NextSeq 550 system that is approaching end-of-life status. This is a Presolicitation notice in accordance with RFO 5.101.
To support the mission of the Armed Forces Medical Examiner System (AFMES) and the Defense POW/MIA Accounting Agency (DPAA), AFMES-DNA OPS with the identification of missing service members from World War II, Korea, Vietnam, and the cold war as part of DoD’s past accounting missions. To meet the National Defense Authorization Act (NDAA) 2010 mandate for 200 annual identifications, AFMES- DNA OPS has developed, validated Next-Generation Sequencing (NGS) methods on Illumina platforms.
Due to the end-of-life status of the current NextSeq 550 systems and a significant increase in mission sample workload, a direct replacement is required. To maintain mission continuity, one (1) Illumina NextSeq 2000 Sequencing System is needed. This will allow for the seamless transfer of existing validated assays and provide the increased throughput necessary for identifying fallen service members.
The NextSeq 2000 is the direct, next-generation replacement for the laboratory's current, end-of-life Illumina sequencers. It offers backward compatibility with the existing validated assays while providing increased throughput (up to 120 Gb), faster run times (approximately 8-42 hours), and higher data quality (2'90% of bases >Q30). This is critical as the NGS sample workload has increased. Furthermore, the NextSeq 2000 offers future scalability, as it can be upgraded to another NextSeq System if mission demands increase further.
Switching to a different manufacturer's system would require complete re-development and re-validation of all sequencing assays, a...
To support the mission of the Armed Forces Medical Examiner System (AFMES) and the Defense POW/MIA Accounting Agency (DPAA), AFMES-DNA OPS with the identification of missing service members from World War II, Korea, Vietnam, and the cold war as part of DoD’s past accounting missions. To meet the National Defense Authorization Act (NDAA) 2010 mandate for 200 annual identifications, AFMES- DNA OPS has developed, validated Next-Generation Sequencing (NGS) methods on Illumina platforms.
Due to the end-of-life status of the current NextSeq 550 systems and a significant increase in mission sample workload, a direct replacement is required. To maintain mission continuity, one (1) Illumina NextSeq 2000 Sequencing System is needed. This will allow for the seamless transfer of existing validated assays and provide the increased throughput necessary for identifying fallen service members.
The NextSeq 2000 is the direct, next-generation replacement for the laboratory's current, end-of-life Illumina sequencers. It offers backward compatibility with the existing validated assays while providing increased throughput (up to 120 Gb), faster run times (approximately 8-42 hours), and higher data quality (2'90% of bases >Q30). This is critical as the NGS sample workload has increased. Furthermore, the NextSeq 2000 offers future scalability, as it can be upgraded to another NextSeq System if mission demands increase further.
Switching to a different manufacturer's system would require complete re-development and re-validation of all sequencing assays, a...
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| Issuing agency | Defense Health Agency |
|---|---|
| Country | United States |
| Category | Government Procurement |
| NAICS | 334516 |
| PSC | 6515 |
| Published | August 03, 2026 |
| Procurement stage | Active solicitation |
| Closed | August 18, 2026 |
| Status | Closed — no longer accepting responses |
| Official source | View original notice |
| Last verified | October 11, 2026 |
Source: U.S. Government (SAM.gov) — public domain. GlobalGov is not affiliated with or endorsed by the United States Government.
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